Journal: Metabolic Engineering Communications

Article Title: Multi-layered metabolic remodeling of Pseudomonas putida for efficient conversion of lignocellulosic sugars to the precursors of advanced aviation fuel

doi: 10.1016/j.mec.2026.e00274

Figure Lengend Snippet: Fed-batch cultivation of PIPAxyl-E3-K3-O15 for isoprenyl acetate production. (a) Residual sugar concentrations and feeding rate over time. The culture was initiated with 13.3 g/L glucose and 6.7 g/L xylose and continuously fed with a solution containing 266.7 g/L glucose and 133.3 g/L xylose. (b) Time-course profile of isoprenyl acetate production. Titers represent the sum of isoprenyl acetate quantified from the aqueous phase, the Durasyn overlay in the bioreactor, and the Durasyn off-gas trap. Cultivations were performed with 1 L medium and 200 mL overlay with sampling approximately every 12 h. Arabinose, salicylic acid, and m -toluic acid were added at OD 600 0.6–0.8 to final concentrations of 2 g/L, 62.5 μM, and 250 μM, respectively.

Article Snippet: Fed-batch cultivation was conducted in a 2 L DASGIP parallel bioreactor system (Eppendorf) with a working volume of 1.2 L. Seed cultures were prepared through a three-step adaptation.

Techniques: Sampling

Journal: Applied Microbiology and Biotechnology

Article Title: Evolutionary engineering of Geobacillus thermoleovorans for growth on adipic acid and 1,4-butanediol

doi: 10.1007/s00253-026-13836-8

Figure Lengend Snippet: One-pot hydrolysis of PBAT by HiC and growth of evolved G. thermoleovorans on the released AA and BDO. The process was conducted in DASbox® mini-bioreactors filled with 100 mL MSM with 25 g L −1 PBAT powder and 5 µM HiC. The starting OD 600 of each strain was 0.05. Samples were taken daily during the whole process, and the OD 600 ( A ), AA ( B ), BDO ( C ), and TA concentration ( D ) were measured. On day 15, after growth stagnated in all reactors, a different pulse was added to each reactor. Symbol shapes and colors represent the bioreactors with their designated pulse: MSM (green circle), water (blue square), 30 mg L −1 iron (purple triangle)

Article Snippet: G. thermoleovorans strains were cultivated in a 350 mL DASbox® mini-bioreactor system (Eppendorf SE, Hamburg, Germany) under the control of the DASware control software, using a working volume of 100 mL.

Techniques: Concentration Assay